Coding

Part:BBa_K4451014:Design

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


ACG-SmR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 587
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 587
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 719
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 587
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 587
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sheffield 2022 designed this part for testing the function of their MutaT7-based directed evolution system ‘rEvolver’. Since this system was designed for use in V. natriegens, BBa_K4451014 has been codon-optimised accordingly. This part is used in the composite ‘MutaT7 Test Cassette’ BBa_K4451021, where streptomycin resistance above that of the spontaneous mutation rate acts as a reporter for cytosine deaminase activity (caused by a C → T transition mutation; see BBa_K4451021 for more information).

Source

References